Description
Principle: This mouse Gp130 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Gp130 has been precoated onto 96-well plates. Standards(NSO, Q23-E617) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Gp130 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Gp130 amount of sample captured in plate.
Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Gp130/IL6ST with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Gp130/IL6ST tissue specificity: Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells.